For implementers, BYOB adds significant complexity. The stream must track pending BYOB requests, handle partial fills, manage buffer detachment correctly, and coordinate between the BYOB reader and the underlying source. The Web Platform Tests for readable byte streams include dedicated test files just for BYOB edge cases: detached buffers, bad views, response-after-enqueue ordering, and more.
Klenerman and Balasubramanian’s great insight was that template DNA could be sequenced by synthesizing a complementary strand of reversible terminators; basically, extending the chain one base at a time and determining the identity of each nucleotide by looking at the color of its fluorophore. In 1998, the pair started a company called Solexa to develop the technology.
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