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For each minus reaction, the purified DNA fragments were incubated together with three of the four dNTPs, meaning each fragment would be extended by DNA polymerase until the missing nucleotide was needed, at which point synthesis would halt.
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McKenzie is one of 120 BAS employees in total who were in Antarctica for the summer season that is now coming to an end. Most, including McKenzie, will return to the UK by the end of May, yet up to 50 will stay for the darkness of winter.